Molecular Biology and biotechnology principles
Molecular Biology and biotechnology principles
In this course students will learn about molecular biology and biotechnology principles.
1. Unit 1
Topics covered in : Lecture 1 , 2 and 3
Recombinant DNA technology
Tools used in Recombinant DNA technology
Isolation of vector gene
Isolation of desired gene
Methods to transfer foreign gene into bacterial cell
Discuss about Restriction enzymes
And about ligases
Vectors like cosmid, plasmid
Production of insulin gene
Applications of recombinant DNA technology
2. Unit 2
Topics covered in Lecture 4
Molecular biology Techniques
Southern blotting technique used for DNA samples
Northern blotting used for RNA samples
3. Unit 3
Topics covered in Lecture 5
Hybridisation technique
Polymerase chain reaction used to amplify the DNA in the sample
Radio immuno Assay - Antigen and antibody specific
Electrophoresis
zone electrophoresis
Gel electrophoresis
Polyacrylamide gel electrophoresis.
Paper electrophoresis.
Principle of electrophoresis.
Used to separate the DNA depending on the size and charge on the molecule.
Gel electrophoresis - is used to separate DNA or RNA.
SDS page used to separate proteins and amino acids
4. Unit 4
Topics covered in Lecture 6 and 7
chromatography
column chromatography
Affinity chromatography.- based on affinity towards ligand and protein .
Ion exchange chromatography.
Cat-ionic exchanger and anionic exchanger
Separation is based on ions present in the protein sample
Gas liquid chromatography. Gas acts as a mobile phase and liquid acts as a stationary phase .gases like inert gases are used and liquid of high boiling point is used in gas liquid chromatography
Paper chromatography . Paper is used and reagent are used to identify the compound present in the sample
principle of chromatography
separation of molecules based on size, charge and molecular weight
Separation of macro molecules based on antigen and antibody interaction
Enzyme and ligand interaction as there is a specific interaction between the antigen and antibody interaction
Summary of the course
Add-on
Course would be added/enhanced based on Q&A form students in to form of additional information
Recombinant DNA Technology , Polymerase Chain Reaction ,Electrophoresis , Chromatography, Blotting technique and ELISA
Url: View Details
What you will learn
- Recombinant DNA Technology or Cloning
- Polymerase Chain Reaction
- Hybridisation and Blotting Techniques
Rating: 4.5
Level: Intermediate Level
Duration: 2 hours
Instructor: Jhanavi Ramanujam
Courses By: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
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