Sterilization and Culture methods detailed

Sterilization and Culture methods detailed

Introduction sterilization

Definition and Methods

Physical Methods - Red Heat

Hot Air Oven

Moist heat and auto clave

Pasteurization

IR radiation

Non IR

Filtration

5 types of Filtration

chemical method- two types of gases

Liquid stage

4 types of Liquid chemicals

Applications

Introduction Culture methods

culture media definition and ingredients

types of media

physical media

semi solid media

solid media

chemical media  3 types

non synthetic media

special media 3 types

Enriched media

selective media

differential media

anaerobic media

Transport media

Antibiotic media

conclusion

Two major contributions to the art of sterilization came in the 1860’s

when the French chemist and microbiologist Louis Pasteur

wrote extensively on how germs cause disease and the English physician, Joseph Lister,

developed a technique that used carbolic acid as a spray to disinfect instruments and so on. (refer video slides all full info)

The first liquid artificial culture medium was created by Louis Pasteur in 1860

Previously bacterial growth on daily materials such as some foods had been observed

Louis Pasteur was a French chemist and microbiologist renowned for his discoveries of the principles of vaccination microbial fermentation and pasteurization

The German physician Robert Koch (December 11 1843 — May 27 1910) is considered the father of modern bacteriology for his work demonstrating that specific microbes are responsible for causing specific diseases

Koch discovered the life cycle of the bacteria responsible for anthrax and identified the bacteria that cause tuberculosis and cholera

Culture media

A microbiological culture medium is a substance that encourages the growth support and survival of microorganisms

Culture media contains nutrients growth promoting factors energy sources buffer salts minerals metals and gelling agents (for solid media)

Different types of culture media are typically divided based on the physical state of the media

Liquid culture media

commonly called broth Solid and semi-solid culture media commonly called agar. (refer video slides all full info)

sterilization and culture media concepts

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What you will learn

• Introduction sterilization
• Definition and Methods
• Physical Methods - Red Heat

Rating: 3.75

Level: All Levels

Duration: 3 hours

Instructor: Sowjanya Chollangi