HPLC-Principle, Instrumentation, Parameters and Applications
HPLC-Principle, Instrumentation, Parameters and Applications
The contents of the Online Course are listed below:
1. Introduction to Author, Contents of HPLC [High performance liquid chromatography].
2. About HPLC.
3. Types of HPLC Techniques and Principle of HPLC.
4. Instrumental requirements – HPLC Pumps, different types of HPLC pumps.
5. Mixing Unit, gradient controller, solvent degassing.
6. Injectors and their types - Septum injectors, Stop flow injectors, Rheodyne injectors.
7. Columns and their types\- Guard Columns and Analytical Columns.
8. Types of Columns - Normal phase columns, Reverse phase columns, Ion exchange columns and Size exclusion columns
9. Detectors and their types.
10. Types of Detectors - UV/Visible detectors, RI detectors, fluorescence detectors, electrochemical detectors, conductivity detectors
11. Types of Detectors - evaporating light scattering detectors, mass detector, Ir detector and optical rotation detector
12. Recorders and Integrators.
13. Parameters used in HPLC, Retention Time, Retention Volume
14. Resolution and Separation factor
15. Theoretical Plates
16. Asymmetric Factor
17. Applications of HPLC
18. Quantitative Analysis
19. Multi-Component Analysis
About the Author:
Author has completed M. Pharmacy and pursuing Ph.D, completed Diploma in Nutrition and having 15 years of teaching experience in reputed Pharmacy Colleges. She has published 30 research papers in various International Journals and review Articles. She also published an Article in “Future of Education after Pandemic”
I have attended seminars and workshops in various reputed colleges and attended Indian Pharmacy Council (IPC).
Previous Courses of the Author are Introduction to Pharmaceutical Analysis and UV Visible Spectroscopy.
HPLC-Principle, Instrumentation, Parameters and Applications
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What you will learn
- Introduction to HPLC
- Types of HPLC
- Instrumental Requirements of HPLC
Rating: 5
Level: Intermediate Level
Duration: 1.5 hours
Instructor: Lakshmi Aswini
Courses By: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
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